Kekik ve mercanköşk diğer yağlara göre daha yüksek antimikrobiyal aktivite gösterirken, her iki mikroorganizmaya karşı en yüksek antimikrobiyal aktiviteyi. Pazarbasi, B., Yilmaz, M., Türkiye Kosullarinda Üretimi Yapilan Spirulina platensis’ in Çesitli Ekstraktlarinin Antimikrobiyal Aktivite Kapasitesinin Belirlenmesi. antimikrobiyal aktivite, antioksidan aktivite, fenolik madde. Özet: Bu çalışmada Türkiye’nin Doğu Akdeniz Bölgesi’nden toplanan Tornabea.

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The concentration of essential oil which had the same turbidity with negative control was determined as MIC and it was corroborated by OD measurement.

Show full item record. Although, they are GRAS, the application of essential oils is limited because of organoleptic changes in food. Intracellular pH and ATP concentration and the release of cell constituents were measured when mustard essential antkmikrobiyal was in contact with E. On the contrary, no antibacterial activity was observed for basil, ginger and fennel essential oils against E.

The MIC values found for microplate assay was always lower that those found in antjmikrobiyal measurement because of its sensitivity. Essential oils can comprise more than sixty individual components.

Inhibitory effect of these selected essential oils were monitored during incubation period 0; 2; 4; 6; 8; 10; 12; 18, 20; 22 and 24 h and the bacterial growth was determined by measuring optical density Antimikdobiyal at nm. H7 and Salmonella typhi was investigated. MBC values of thyme and origanum essential oils against E.

Browsing by Subject “Antimikrobiyal Aktivite”

The obtained results are more sensitive than agar dilution method. Leakage of ions and other cell contents can aktivte occur. However, origanum essential oil showed the highest inhibitory activity against both bacterial species tested. In broth dilution studies a number of different techniques exist the most used methods are that of optical density OD turbidity measurement and the enumeration of colonies by viable count.

Browsing by Subject “Antimikrobiyal Aktivite”

A number of essential oil components has been identified as effective antibacterials, e. Microdilution method was used to determine MIC. Dilution methods are usually used to obtain minimum inhibitory concentration MIC and minimum bactericidal concentration MBC. The obtained inhibition diameters of essential oils against E.

Extensive loss antimikrobiyl cell contents or the exit of critical molecules and ions will lead to death. However, most studies investigating the action of whole essential oils against food spoilage organisms and food borne pathogens agree that, generally, essential oils are slightly more active against gram-positive than gram-negative bacteria.


Essential oils, known as volatile oils, are complex mixtures of aromatic and volatile constituents which are obtained by such as distillation, cold pressing and maceration.

Please use this identifier to cite or link to this item: The MIC was determined by comparing the turbidity of the essential oil added well with the turbidity of negative control added well.

Gram-negative bacteria are less sensitive to the antimicrobials because of the lipopolysaccharide outer membrane of this group, which restricts diffusion of hydrophobic compounds.

Also, the extracellular ATP concentration increased and a reduction of the intracellular pH was observed in both bacteria. MBC is defined as concentration where In aktifite study, dillweed, cardamon, basil, thyme, cumin, origanum, fennel and ginger essential oils were studied for their antimicrobial activity against Escherichia coli and Staphylococcus aureus by agar well diffusion method.

Results showed that planting space had significant effect on plant diameter and very significant effect on other measured parameters except oil content, which was not significant. However outcome akticite a test can be affected by factors such as the method used to extract the essential oil from plant material, the volume of inoculum, growth phase, culture medium used, pH of the media and incubation time and temperature.

Tests of antimicrobial activity can be classified as diffusion and dilution methods. Diffusion methods can akivite classified as disc diffusion and agar well diffusion methods while dilution method can be classified as agar dilution and broth dilution methods. However, the increased demand for safe and natural food, without chemical preservatives, provokes many researchers to investigate the antkmikrobiyal effects of natural compounds. The activity of dillweed, cardamon, thyme, cumin and origanum essential oils against E.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated. However, the stereochemistry, lipophilicity and other factors affected the biological activity of these compounds which might be altered positively or negatively by slight modifications. Preservation of food materials from spoilage, mainly by microbial activity, during production, storage and marketing is an important issue in the food industry.


Time of harvest had significant effect on yield of fresh herbage, content of oil and content of carvacrol. In the second part of this study 4 essential oils thyme, cumin, cardamon and origanum essential oils which showed antimicrobial activity against E.

Hydrophobicity of essential oils and their components enables them to partition in the lipids of the bacterial cell membrane, disturbing the structures and rendering them more permeable. In this method obtained MIC values of E.

The apparent antimicrobial efficacy of plant origin antimicrobials depends on factors such as the method of extracting essential oils from plant material,the part of a plant where the essential oil is extracted,type of solvent, volume of inoculum, growth phase, culture medium used, concentration of essential oil, temperature, oxygen and intrinsic or extrinsic properties of the food such as pH, fat, protein, NaCl, and physical structure of food. Therefore comparison of published data is complicated.

A significantly higher release of cell constituent was observed when both bacteria cells were treated with mustard essential oil. Cumin and cardamon showed the lowest antimicrobial effect against E.

To achieve this purpose the food industry has used different physical and chemical methods which diminish microbial growth or inhibit microorganisms and prevent or delay.

In a study, how mustard essential oil affected the cell membrane of Escherichia coli O Its effect on other parameters was very significant except dry herbage and oil yield.

There are also new concerns about chemical preservatives due to increasing occurrence of many respiratory illnesses. Thyme and origanum essential oils showed higher antimicrobial activity than the other essential oils.

The maximum yield of dry and fresh herbage, yield and content of oil and thymol yield were obtained beginning of blooming stage. In this study the results show that gram-negative E. MIC is defined as the lowest concentration of the essential oil which inhibits bacterial growth. Applying different methods to determine antimicrobial activity of an essential oil cause to be obtained different results.