API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.
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An organism unable to utilize this substrate results in the accumulation of succinic acid and the organism cannot grow.
When Actinobacillus or Pasteurella sp. Green or blue is positive reaction. Green should be regarded as a negative reaction. If further speciation is required for Yersinia spp. These strips are intended for in vitro use only; for use by qualified laboratory personnel using aseptic techniques and established precautions against microbiological hazards.
Evaluate within 2 minutes of the addition of the reagent. Organism identification is based on pH change and substrate utilisations as established by published reference methodologies 4,6,8,9.
Using a sterile pipette or balerie bottle, overlay the substrates underlined on the holding tray with sterile mineral oil, i. H 2 S is produced from thiosulphate. The following chart gives the expected results on the Microbact System after an hour incubation: Reactions obtained galeerie the Microbact System may differ from published results using other substrate formulations. Utilisation of Na malonate at the same time that ammonium sulphate is utilised as the nitrogen source produces sodium hydroxide resulting in increased alkalinity and a blue colouration.
Warning and precautions 1.
MB, Microbact GNB 12A () | Oxoid – Product Detail
The 12A 12E strip should be read at hours. Acetoin is produced from glucose indicated by the formation of a galeri complex after the addition of alpha-naphthol and creatine.
The results should be interpreted as follows:. All systems should be read after 48 hours for the identification of Miscellaneous Gram-negative bacilli. Set up proceedure Isolation An hour pure culture of the organism to be identified must be obtained. Kit presentation Each kit contains the following: Each group of three reactions produces a single digit of the code.
API PANEL READING
The reactions are evaluated as positive or negative by comparing them with the colour chart. Quality control The overall performance of the system should be monitored by testing appropriate control strains. Identification of Bacteria by Computer: Remove the strips or tray from the incubator, peel back the sealing tape.
Using the results obtained, the indices of the positive reactions are circled. Evaluate 15 to 30 minutes after the addition of reagents. These can be purchased individually or as a set by ordering product code MB Microbact Biochemical Identification Kits. Record all positive results. Using a sterile Pasteur pipette, transfer one drop of the peptone water culture into the appropriate volume gaperie Procedure Chart of sterile saline solution 0.
Well 1 lysine Well 2 ornithine Well 3 H 2 S. The sum of these indices in each group of three reactions forms the code number. A small amount of zinc powder should be added to those wells which exhibit a yellow colour after the addition of the nitrate reagents.
Identification of Pseudomonas aeruginosa with the APIE system.
Prolonged incubation, insufficient incubation, improper filling of wells, or inadequate inoculum may lead to false results. The following organisms are recommended for independent laboratory assessment. General Aspects and Perspectives. The 12B strips gallerie be used alongside the 12E, but in a 220e tray. The interpretation of mathematically calculated identification results requires trained clinical personnel who should use judgement and knowledge in conjunction with the following information before accepting the ID of an organism: Each strip consists of 12 different biochemical substrates.
Material Safety Data Sheet. Liquefaction of gelatin by proteolytic enzymes diffuses the black pigment. Some bacterial strains may have atypical biochemical reactions due to unusual nutritional requirements or mutations and may be difficult to identify. The pH shift indicated by bromothymol blue caused ga,erie formation of the specific amine putrescine is greater than that caused by lysine decarboxylation.